NYSTATIN SWISH AND SWALLOW DURATION TRIAL

Equitability assumes a value between 0 and 1 with 1 being complete evenness. Bacterial diversity within each sample (alpha diversity) will be computed as the Shannon Index. USearch will be used to identify specific bacteria/fungi or operational taxonomic units using the GreenGenes and UNITE databases of reference sequences. Sequence reads will be assessed for quality using Quantitative Insights into Microbial Ecology software. Absolute abundance of bacteria and fungi in each sample will be determined by quantitative real-time PCR using 16s and ITS primers, probe and cloned plasmid standards.

The bacterial 16S rRNA V1-V3 hypervariable region and fungal ITS region will be amplified and sequenced on an Illumina MiSeq. Genomic DNA from clinical samples will be extracted. We will use established methods to perform the oral microbiome sequencing and related bioinformatics analysis. Detailed inclusion and exclusion criteria and methods see below. mutans) will be monitored immediately (within 7 days) and 3-months following the completion of the 1-week Nystatin application. The oral microbial changes including fungal and cariogenic bacteria (e.g. All study participants will receive treatment for oral candidiasis using nystatin suspension (Brand name: Mycostatin), and be instructed to rinse the mouth with (6ml of 600,000 U/mL) nystatin suspension, followed by spitting out the suspension, at the frequency of four times per day, for a period of 1 week. Epstein at the Department of Oral Diagnosis and Department of Microbiology and Immunology at the University of Washington in 1980). All study participants will have positive oral Candida detection with sufficient oral Candida burden to meet the laboratory criteria for a diagnosis of oral candidiasis (≥ 400 CFU/mL of salivary Candida, a standard that was established by Dr.

NYSTATIN SWISH AND SWALLOW DURATION TRIAL

This is a prospective, single-arm clinical trial of patients diagnosed with oral candidiasis.ĭesigned clinical study. Why Should I Register and Submit Results?.

Nystatin swish and swallow duration